Fig. 4
Expression of CD31, α-SMA and Snail in the kidney. a CD31 and α-SMA mRNA expression was quantified by real-time PCR and normalized to β-actin mRNA expression in the kidney tissues of sham-operated rats treated with the control adenovirus at postoperative week 13 (sham), 5/6 Nx rats treated with the control adenovirus (5/6 Nx), and 5/6 Nx rats treated with Ad-netrin-1 (5/6 Nx + netrin-1). b A representative western blot of the proteins extracted from the three experimental groups, indicating the level of CD31 and α-SMA protein expression relative to β-actin. c Relative expression of Snail mRNA was determined by realtime RT-PCR and normalized to β-actin mRNA (left panel). Snail protein levels were assessed by Western blot (right panel). All data are expressed as mean ± SEM; *, P < 0.05 vs. sham; #, P < 0.05 vs. 5/6 Nx