Fig 5

YAP expression was reduced in RhoA or mDia knockdown podocytes. a Con and Con-siRNA were short for blank control and control siRNA respectively. Confocal images of podocytes showing expression of YAP (green), Phalloidin-stained stress fiber (red) and DAPI-stained nuclei (blue). Compared to con-siRNA, nuclear and cytoplasmic YAP and stress fiber were obviously reduced in RhoA-siRNA or mDia-RNA treated podocytes. Scale bar = 50 μm. b & d WB results show that nuclear YAP protein expression was reduced in RhoA-siRNA or mDia-RNA treated podocytes. c & e Total YAP protein expression was reduced in RhoA-siRNA or mDia-RNA treated podocytes. All results were expressed as fold YAP of RhoA-siRNA or mDia-RNA group in relation to con-siRNA group normalized to Histone or GAPDH. There is no significant difference between control group and con-siRNA group. Data were from at least three independent experiments. * P < 0.05 vs con-siRNA group