Fig. 1

Characterization of the PB insertion in Robo2 mutant mice. a Position of the PB insertion. The PB integration site in Robo2 ^PB/PB mice mapped to the first intron of Robo2 on mouse chromosome 16, nucleotide 74379378. Diagram shows the genomic structure of the Robo2 gene with exons (vertical lines) and introns (transverse lines). Gene-specific primers are indicated. b Disruption of Robo2 transcript in Robo2 mutants. Robo2 transcripts were analyzed by quantitative PCR using the indicated primers P1/P2. The cDNAs from whole embryos at E15.5 were amplified as templates. GAPDH was used as an internal control. **P < 0.01, n = 3 per group. c Western blots were performed on the brain and kidneys from wild-type and Robo2 ^PB/PB mice. The ROBO2 expression level decreased in Robo2 ^PB/PB mice. d Expression of ROBO2 in the kidneys. a and c :expression of ROBO2 in the kidneys from Robo2 ^+/+ mice; b: expression of ROBO2 in the kidneys from Robo2 ^PB/PB mice; d: expression of ROBO2 in the kidneys from Robo2 ^PB/PB mice with VUR; e: expression of ROBO2 in the kidneys from Robo2 ^PB/PB mice with renal dysplasia