Fig. 1

Generation and characterization of Ksp-Keap1 ^-/- mice. a Ksp-Cre mice were crossed with Keap1 ^f/f mice to generate Ksp-Keap1 ^-/- mice. b Mice were genotyped to confirm the presence of Cre and floxed Keap1 allele using Cre and flox specific primers. c Ksp-Cre mediated deletion of Keap1 exon 2 and 3 resulted in a truncated allele (288 bp) in comparison to WT allele (2954 bp). d mRNA analysis of Nrf2 targets showed increased expression on Nqo1 (p ≤ 0.0001), Gclm (p ≤ 0.05) and Gclc (p ≤ 0.001) but reduced expression of HO-1 (p = ≤0.001) and Nrf2 (p = ≤0.001). b Lane 1 = 100 bp DNA ladder, lane 2 = 324 bp internal positive control and 100 bp Cre and lane 3 = 383 bp Keap1 floxed. c Lane 1 = 1 kb DNA ladder, lane 2 = 2954 bp WT allele (Keap1f/f), lane 3 = 288 bp truncated allele (Ksp-Keap1 ^-/-) and lane 4 = 100 bp DNA ladder