Fig. 5

An association of CLU deficiency with worse cellular infiltration after 30 days of IRI. As described in Fig. 4, the contralateral right kidneys were harvested on day 29, and the injured left kidney on day 30 after IRI. The sections (two per each kidney) of both kidneys (the left and right from KO or WT) were stained with HE for the examination of the content of mononuclear infiltrates, labeled by dark/black nuclear staining. a A typical microscopic image of renal cortex in each group (KO: CLU null kidneys; WT: WT kidney sections). Left column: tubule damage and cellular infiltration of the injured left kidneys; right column: normal tissue architecture of the contralateral right kidneys. G: glomerulus; PT: proximal convoluted tubule; DT: distal convoluted tubule; IA: interlobular artery; and IV: interlobular vein. b The infiltration was semi-quantitatively scored in at least 20 randomly selected views in two separate sections of each injured left kidney, and was presented in average per view. Data are presented as mean ± standard derivation (SD) of each group. KO group vs. WT control: P < 0.0001 (two-tailed t-test, n = 8)