CCL18 synergises with high concentrations of glucose in stimulating fibronectin production in human renal tubuloepithelial cells

Table 1 MTT assay of HK-2 cells in different experimental conditions and with or without co-stimulation with recombinant cytokines rCCL18 or rMCP-1 for 48 h

Condition with 0 ng/ml or 20 ng/ml of recombinant cytokine stimulation	MTT assay for HK-2 cells stimulated without or with rCCL18 Mean ± SD	MTT assay for HK-2 cells stimulated without or with rMCP-1 Mean ± SD
N0	0.664 ± 0.024	0.404 ± 0.072
A0	0.331 ± 0.040 **	0.107 ± 0.029 *
M0	0.692 ± 0.073	0.390 ± 0.005
H0	0.843 ± 0.131	0.603 ± 0.033
N20	0.633 ± 0.085	0.517 ± 0.059
A20	0.353 ± 0.042 **	0.263 ± 0.199
M20	0.590 ± 0.113	0.183 ± 0.008
H20	0.758 ± 0.057	0.365 ± 0.178

Abbreviations: N physiological glucose, A glycated albumin, M mannitol, H high glucose
Legend to Table 2: The results from two independent experiments are shown. MTT assay were carried out for 3 wells with each of the cell culture conditions. The results are presented as mean ± SD. The data were tested by Analysis of Variance with Bonferroni’s correction for multiple comparisons. **p < 0.01, * p < 0.05, in comparison to the cells culture in normal glucose concentration

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