Fig. 3
From: Sustained hyperosmolarity increses TGF-ß1 and Egr-1 expression in the rat renal medulla
Effect of chronic increase in medium osmolarity on viability and TGF-ß expression of IMCD cells. a Chronic hyperosmolarity significantly reduced the viability of IMCD cells (n = 8/group). b The expression of TGF-ß, however, increased dramatically both at mRNA and protein level (n = 4/group). c Accordingly, immunocytochemistry depicted significant TGF-ß staining at 600 mOsm and even stronger staining at 900 mOsm (n = 3/group). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (one-way ANOVA with Tukey’s post-hoc test)