Fig. 6
Immunocytochemistry for αSMA in MDCK cells. MDCK cells were cultured in DMEM medium supplemented with 10% FBS at 37 °C with 5% CO2. a Unstimulated MDCK cells (Control), (b) MDCK cells incubated with the IDO inhibitor 1-methyl-D-tryptophan (MT; 1 mM), (c) TGF-β 1-stimulated MDCK cells, and (d) TGF-β 1-stimulated MDCK cells treated with MT. The quantitative analysis demonstrated that the number of αSMA+ cells was increased by TGF-β 1 and the treatment with MT potentiated this effect (e). The conditions were performed in triplicate. *p < 0.05 vs. Control