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Table 1 Characterization of EMT in the UUO model

From: Indoleamine 2, 3-dioxygenase (IDO) increases during renal fibrogenesis and its inhibition potentiates TGF-β 1-induced epithelial to mesenchymal transition

  SHAM CL UUO
Renal fibrosis Interstitial area (%) 0.3 ± 0.1 0.9 ± 0.1 13.4 ± 2.6
Macrophages Interstitial area (cells/field) 6.2 ± 0.8 16.0 ± 2.7 75.2 ± 12.6
E-cadherin Tubular % positive tubules 20.8 ± 5.4 21.1 ± 11.6 02.7 ± 1.1
αSMA Tubular % positive cells 27.2 ± 6.5 35.8 ± 0.90 61.4 ± 4.2
Interstitial positive cells/field 17.7 ± 7.1 74.5 ± 23.5 368.8 ± 45.8
Vimentin Tubular % positive cells 02.0 ± 0.5 02.5 ± 0.50 033.8 ± 2.7
Interstitial positive cells/field 13.0 ± 0.9 17.0 ± 1.20 141.3 ± 8.8
TGF-β 1 Relative expression 1.0 ± 2.2 6.8 ± 0.3 14.7 ± 0.1
  1. UUO kidneys presented increase of interstitial fibrosis, macrophage infiltrating, markers of mesenchymal cells, and TGF-β1 expression. Renal fibrosis was evaluated by Masson’s trichrome-stained interstitial area. Interstitial macrophages were identified by immunohistochemistry. EMT markers were also analyzed by immunohistochemistry. Real-time PCR was used to analyze TGF-β1 expression in the renal tissue. Data are expressed as the mean ± SEM. p < 0.001 versus SHAM and CL; p < 0.0001 versus SHAM and CL