Table 1 Primers used for adenylyl cyclase isoform mRNA detection (5′ to 3′)
| Â | Forward primer | Reverse primer | Produce size (bp) |
|---|---|---|---|
AC1 | GCTGTTCGTGGTCACCAATGTCCG | GAGCGCTCTGTCAAGATCCGCACG | 402 |
AC2 | CAACACTGTCAACGTCGCTAGTAG | GAGCACGTACGTAATCAAGACGAAG | 310 |
AC3 | CCAGTCACTGGAGGTGAAGATGAA | GATGCTGACATTCTCGTGCCGGTA | 179 |
AC4 | CACCATGGTGGAATTTGCAGTGGC | GAGGATCTTCGAAGAGGGGAGCTC | 387 |
AC5 | CAATACAGTGAATGTGGCCAGCCG | CAGCAAAGGCAGAAGTTGCTTCTG | 336 |
AC6 | GGAAAGTAGATCCTCGCTTCG G | ACCGAGAAGATTCCAACCGC | 262 |
AC7 | GTACACTCCAGGCCATCTCG | TCCGCAGGAAAACAGAGCAT | 261 |
AC8 | GCATACAAGAGATCAACAAGCATTC | CTGGTCCTTCAGGATAAGGTAGGT | 212 |
AC9 | CATTGTGATGTCCCCCTTGGA | GTAGGGGATGATGTTCCGCA | 283 |