Fig. 2

Representative findings of tubules in MCNS patients in the non-AKI group. a-b. PAS staining (a) and staining of vimentin (b) in consecutive sections. Mesangium and vessels are positive for vimentin, but tubules are negative for vimentin. Note, tubules with vacuoles (a-b, asterisks) and hyaline droplets (a-b, arrow head) do not express vimentin. c-d Staining of vimentin (c) and staining of Ki67 (d) in consecutive sections. Small groups of simplified tubules include cells with vimentin-positivity in basal or cytoplasmic patterns, which are in the vicinity of a glomerulus (c). + indicate the distal tubule from a morphological aspect (c). Ki67 is expressed in some tubular cells among the vimentin-positive tubules (d) (g: glomeruli; original magnification ×200).