Fig. 4
a-d: HIF-2α renal expression in MORG1+/+ and MORG1+/− mice treated with LPS. a Protein expression of HIF-2α in kidney sections. Reduction of MORG1 expression increased basal and LPS dependent on HIF-2α tubular/peritubular protein expression. Representative images are shown. Original magnification 200×. Bars correspond to 50 μm. G - glomerulus. b HIF2-α renal immunohistology evaluation. The number of the positively stained HIF2-α cells per field was counted and is graphically presented. N = 6 per group, MORG1+/+ saline vs. MORG1+/− saline treated mice, p < 0.001; for other pairwise comparison as shown on the graph the significance was p < 0.001. ***p < 0.001. c Determination of renal Hif-2α mRNA expression using real-time PCR analyses. N = 6 per group, MORG1+/+ NaCl vs. MORG1+/− NaCl, p = 0.018. *p < 0.05. d Real-time PCR analyses of erythropoietin (Epo) kidney mRNA expression during endotoxemia. The renal expression of Epo was analysed as a representative HIF-target gene. The basal levels of Epo were slightly, but not significantly increased in MORG1+/− mice. N = 6 per group