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Table 1 PCR primer sequences

From: Phosphate stimulates myotube atrophy through autophagy activation: evidence of hyperphosphatemia contributing to skeletal muscle wasting in chronic kidney disease

Gene name

Forward primer (5′- 3′)

Reverse primer (5′- 3′)

LC3

CACTGCCGCCCTAAAGGTTAC

GACCGCTCCGTCAGAATGTTG

p62

TCCCTGTCAAGCAGTATC

GATCTTTGCACCAGTCTC

BMP-2

CTGTCCCTACTGATGAGTTTC

CTAACCTGGTGTCCAATAGTC

OPN

TATCCCGATGCCACAGATG

GTGTTTCCACGCTTGGTTC

Osx

CAAGGCAGTTGGCAATAG

AATGGGCTTCTTCCTCAG

Pit-1

AAACGGAGGACAACTATCAG

TACCACAGGCAAGTCTTATC

ALP

CCTTCCGGTATTGACTGTG

GAAACCTGACCCTGAAGTG

Cbfa-1

ACTTCGTCAGCGTCCTATC

CATCAGCGTCAACACCATC

MGP

AAAGCCCAGGAAAGAGTC

TGAAGTAGCGGTTGTAGG

  1. Abbreviations: LC3 Microtubule-associated protein 1 light chain 3, ALP Alkaline phosphatase, BMP-2 Bone morphogenetic factor-2, Cbfa1 Core-binding-factor α1, MGP Matrix GLA protein, OPN Osteopontin, Osx osterix
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BMC Nephrology

ISSN: 1471-2369

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