Fig. 7
Nitrotyrosine IHC of kidneys from STZ-treated and db/db mice. Panel I: Nitrotyrosine IHC from control kidneys (a, d) and at 4 weeks (b, e)and 12 weeks (c, e) following STZ treatment. IHC staining for nitrotyrosine is absent in the controls but is detectable at 4 weeks and further increased at 12 weeks. Nitrotyrosine IHC staining is most prominent within the cortex as compared to the medulla. (a-c X10; D-F X100). Panel II: Nitrotyrosine IHC of kidneys from control db/m (a, c) and with 16 week old db/db kidneys (b, d). Nitrotyrosine IHC staining is concentrated in the cortex of the 16 week old db/db kidneys and is not detected in the age-matched db/m controls. (a, b X 10; C,D X100). Panel III: Nitrotyrosin IHC score is correlated with two isoforms of MMP-2 in STZ and control kidneys. In db/m and db/db kidneys, NTT-MMP-2 is only correlated with nitrotyrosine ICH score. (R, correlation coefficient; CI, 95% confidence interval; ICH, immunohistochemistry)