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Fig. 4 | BMC Nephrology

Fig. 4

From: Monitoring suPAR levels in post-kidney transplant focal segmental glomerulosclerosis treated with therapeutic plasma exchange and rituximab

Fig. 4

Immunostaining of podocyte AP5 activity To look at the effect of post transplant FSGS patient sera on cultured human podocyte αvβ3 integrin activity, podocytes were immunostained with AP5 antibody after respective overnight treatments. While sera from normal subject (HC) generated only minimum amount of AP5 signal, before TPE treatment (PreTPE) sera harvested from post transplant FSGS patients induced AP5 activity apparently in podocytes, which could be blocked by co-incubation with anti-human suPAR antibody. In contrast, the enhanced podocyte AP5 activity was abolished with the post-TPE treatment sera (PostTPE) collected from therapy responder but not with those post-TPE sera obtained from non-responder

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BMC Nephrology

ISSN: 1471-2369

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