Fig. 2

a-h Images from the first biopsy. a Endocapillary proliferative glomerulonephritis (H&E 400x). b Ultrastructural examination with electron dense immune-type deposits with lack of organized substructure (25,000x). c Direct immunofluorescence study shows bright granular IgG3. d Weak IgG1 e Negative IgG2. f Negative IgG4. g Bright kappa. h Negative lambda staining (all IF images 400x). i-m Images from second biopsy. i Mild mesangial expansion and hypercellularity (H&E 400x). j IgG staining shows absence of granular staining (400x). k Kappa trace staining. l Lambda negative (both 400x). m No electron dense deposits on ultrastructural examination (6000x). n-o Images from third biopsy. n Changes of transplant glomerulopathy (H&E 400x). o Ultrastructural examination with subendothelial widening, electron lucent amorphous material and entrapped non-specific electron densities (30,000x)