Fig. 1

Slc3a1 transcripts and rBAT protein expression are lost in male Slc3a1 knockout mice. Slc3a1^−/− mice demonstrate loss of Slc3a1 RNA (a) but retention of Slc7a9 RNA (b) in kidney and small intestine. Bladder and liver are provided as negative controls. Shown are the averages of two independent experiments done in triplicate (mean ± SD). (c) Representative Western blot of rBAT from wild type and Slc3a1^−/− mice. Immunofluorescence was used to evaluate for rBAT expression in wild type (d) and male Slc3a1^−/− mice (e). Knockout animals demonstrate loss of rBAT expression in the proximal tubule