Fig. 2

Renal biopsy findings. Light microscopy. The glomerulus shows cystic dilatation of Bowman’s capsule and atrophy of the glomerular tuft, (1A) (periodic acid-Schiff (PAS) stain: original magnification × 100) and (1B) (PAS stain: original magnification × 400). Type IV collagen staining. Immunofluorescence study was performed to evaluate the expression levels of type IV collagen. FITC-conjugated-anti α5 chain is observed in the glomerular basement membrane, part of the tubular basement membrane, and Bowman’s capsular basement membrane (1C). In addition, Texas Red-anti α2 chain revealed the renal basement membrane structure (1D). Basement membranes where two fluorescence were present appear green to yellow (1E) (original magnification × 400). Fluorochrome-conjugated anti collagen IV cocktail for Alport’s syndrome was used (Cosmo Bio Ltd. Co., Tokyo, Japan). Electron microscopy. Morphological abnormalities, apart from thinning of the glomerular basement membrane, were not found; there were no electron dense deposits (1F; original magnification × 3000 and 1G; original magnification × 10,000)