Fig. 5

Overexpression of miRNA mimics inhibits luciferase reporter activity in HK-2 cells. a The schematic luciferase reporter constructs. A mutant construct was made by replacing four nucleotides in the miRNA seed binding site of target gene 3’UTR. Mutated nucleotides were marked in gray and underlined. The luciferase reporter assay of ITGB8 (b) and CALM3 (c) 3’UTR reporter in HK-2 cells at 48 h after transfection. EV: empty vector; WT: wild-type; Mut: mutant; NC: scrambled negative control transfection.*p < 0.05 versus NC. Data represent mean ± SEM for at least three independent experiments