Next generation sequencing based assessment of the alloreactive T cell receptor repertoire in kidney transplant patients during rejection: a prospective cohort study

Table 1 Schematic diagram of the patient’s timeline (adjusted from SPIRIT guidelines for trial protocols)

Timepoint	Study period
	Enrolment	Biopsy			Close-out
	Transplantation	For cause	M3	M12	M24
Enrolment:
Eligibility screen	X
Informed consent	X
Interventions:
PBMC collection	X	X	X	X
Kidney Biopsy		X	X	X
Assessments:
TCR repertoire sequencing of unstimulated PBMCs	X	X	X	X
TCR sequencing of Alloreactive T cells after MLR	X
Phenotypic analysis of peripheral T cell populations	X	X	X	X

M3 Month 3, M12 Month 12, MLR Mixed lymphocyte reaction, TCR T cell receptor, PBMC Peripheral blood mononuclear cells

ISSN: 1471-2369