Fig. 3

Binding assay of CFH to C3b. Inhibition of the interaction between CFH and C3b as measured by ELISA. The anti-CFH autoantibodies purified from our patient and the aHUS patient, and the total IgG from three healthy controls were analysed individually. The percentage of inhibition was calculated by defining the average optical density (OD) value obtained in the presence of each healthy control IgG to be 1. The purified anti-CFH autoantibodies from our patient inhibited the interaction between CFH and C3b in a dose-dependent manner. Each assay was performed three times, and the data are shown as the mean ± SD. Notes: P: Our patient; aHUS: the aHUS patient with anti-CFH autoantibodies; HC: healthy controls