Table 1 Immunohistochemistry specifications
From: Renal expression of cytokines and chemokines in diabetic nephropathy
Primary antibody | Supplier | Clone or code | Antigenic recovery | Concentration |
|---|---|---|---|---|
Anti-eotaxin monoclonal antibody | Thermo Fisher Scientific | 43,911 | Citrate pH 6.0 | 1:100 |
Anti-MIP-1α (macrophage inflammatory protein-1α) (CCL3) polyclonal antibody | Thermo Fisher Scientific | PA5–32496 | Citrate pH 6.0 | 1:1600 |
Anti-IL-4 (interleukin-4) polyclonal antibody | Thermo Fisher Scientific | PA5–25165 | Citrate pH 6.0 | 1:1300 |
Anti-IL-8 (CXCL8) polyclonal antibody | Thermo Fisher Scientific | PA5–79113 | Citrate pH 6.0 | 1:1400 |
Anti-IL-10 polyclonal antibody | Thermo Fisher Scientific | PA5–79457 | Citrate pH 6.0 | 1:1200 |
Anti-TNF-α (tumor necrosis factor-α) monoclonal antibody | Thermo Fisher Scientific | 2C8 | Citrate pH 6.0 | 1:1200 |
Anti-TNF Receptor I (tumor necrosis fator receptor-1) monoclonal antibody | abcam | H398 | Citrate pH 6.0 | 1:300 |
Anti-IL-1β polyclonal antibody | Novus Biologicals | NBP1–19775 | Citrate pH 6.0 | 1:40 |
Anti-IL-6 polyclonal antibody | abcam | ab6672 | Citrate pH 6.0 | 1:300 |