Fig. 1

Kidney tissue expressed APOL1 splice variants. a, b APOL1 splice variants were cloned from differentiated, immortalized human podocytes by TA-cloning. The exons of each of the six mRNA splice variants are shown (a), as are the three predicted protein isoforms following signal sequence cleavage (b). Exon lengths are not drawn to actual size. c, d APOL1 splice variant expression in human tissues was analyzed by using a human cDNA library. RT-PCR was performed with two sets of primers as shown by arrows in the above exon schema. APOL1 splice variant TA-vectors were used for PCR positive controls. The major splice variant V1, encoding isoform A, is seen in all examined tissues (c). Human lung and kidney expressed the APOL1 splice variant V2–3 (d; lower band), encoding protein isoform B3