Biobanking for glomerular diseases: a study design and protocol for KOrea Renal biobank NEtwoRk System TOward NExt-generation analysis (KORNERSTONE)

Table 3 Overview of stored samples per participant in the KORNERSTONE

Biospecimen	Stored vial	Volume and amount	Storage temperature
Serum	1.8 ml cryogenic vial	Adults: 300 μl × 5 vials	≤ − 196 °C
Serum	1.8 ml cryogenic vial	Children: 100 μl × 4 vials	≤ − 196 °C
Plasma	1.8 ml cryogenic vial	Adults: 300 μl × 5 vials	≤ − 196 °C
Plasma	1.8 ml cryogenic vial	Children: 100 μl × 4 vials	≤ − 196 °C
Genomic DNA	1.7 ml microcentrifuge tube	Adults: Total more than 100 μg, 3 vials	≤ − 80 °C
Genomic DNA	1.7 ml microcentrifuge tube	Children: Total 30 μg, 2 vials	≤ − 80 °C
Urine	1.8 ml cryogenic vial	Adults: 1 ml × 5 vials	≤ − 80 °C
Urine	1.8 ml cryogenic vial	Children: 200 μl × 5 vial	≤ − 80 °C
Buffy coat	1.8 ml cryogenic vial	Adults: 200 μl × 2 vials	≤ − 80 °C
Buffy coat	1.8 ml cryogenic vial	Children: 100 μl × 2 vials	≤ − 80 °C
Stool DNA	1.7 ml microcentrifuge tube	100 μl, 1 vial	≤ − 80 °C
Stool	30 ml container	1 g, 2 bottles	≤ − 80 °C
Microdissected glomerulus (cDNA)	1.7 ml microcentrifuge tube	Total 70 ng × 2 vials	≤ − 80 °C
Microdissected tubulointerstitium (cDNA)	1.7 ml microcentrifuge tube	Total 100 ng × 2 vials	≤ − 80 °C

ISSN: 1471-2369