Fig. 2

NR1 knockdown partially reversed the increased expression levels of fibrotic markers in UUO kidneys. a Masson’s trichrome staining of kidneys, immunohistochemical staining of fibronectin, α-SMA, and S100A4 (A1), and quantitative analysis (A2) from the control (Con) group, UUO group, UUO + NR1-targeting shRNA-transfected (NR1-sh) group, and UUO + scrambled shRNA transfected (Scr-sh) group. b Western blot analysis of fibronectin, COL1A1, α-SMA, and S100A4 COL1A1 (B1) and quantitative analysis (B2) (n = 6). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 versus the Con group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus the UUO group; ^△△P < 0.01, ^△△△P < 0.001 versus the UUO + NR1-sh group. Representative images from each group (original magnification: × 200; scale bar = 100 μm)