Table 1 Gene specific-forward and reverse primer sequences
Primer | Sequences (5’->3’) | PCR Product length |
|---|---|---|
Cas9: F | ACATCTTCAATGGGACCGGC | 85 bp |
Cas9: R | TCTTTCTGCTCACCACCACAG | |
GAPDH: F | AAAGAGATGCTGAACGGGCA | 100 bp |
GAPDH: R | ACAAGGGAAACTTGTCCACGA | |
Bcl-2: F | GGAGGATTGTGGCCTTCTTT | 100 bp |
Bcl-2: R | GTCATCCACAGAGCGATGTT | |
Ngal: F | TGAACTGAAGGAGCGATTCG | 86 bp |
Ngal: R | ATTGGTCGGTGGGAACAGA | |
Kim-1: F | ACTCCTGCAGACTGGAATGG | 214 bp |
Kim-1: R | CAAAGCTCAGAGAGCCCATC | |
CAT: F CAT: R | GCGAATGGAGAGGCAGTGTA GAGTGACGTTGTCTTCATTAGCACTG | 625 bp |
GPx: F GPx: R | CTCTCCGCGGTGGCACAGT CCACCACCGGGTCGGACATAC | 290 bp |
Cu-Zn SOD: F | GCAGAAGGCAAGCGGTGAAC | 447 bp |
Cu-Zn SOD: R | TAGCAGGACAGCAGATGAGT |