Fig. 2

PA induces the activation of NLRP3 inflammasome and oxidative stress in MPC5 cells. Cells were treated with 150µM PA for 24 h. a, Western Blot analysis of NLRP3, procaspase-1, cleaved caspase-1, and ASC. *** p < 0.001 vs. the control group. b, The mRNA levels of NLRP3 inflammasome-related proteins were detected by qRT-PCR analysis. ** p < 0.01, *** p < 0.001 vs. the control group. c, ELISA assays were performed to measure the contents of IL-18 and IL-1β in cell culture supernatants. *** p < 0.001 vs. the control group. d. ROS production was assessed using a fluorometry assay. *** p < 0.001 vs. the control group. Data are presented as mean ± SEM from three independent experiments