Fig. 4From: Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathwayThe knockdown of adiponectin exacerbates intracellular inflammation and apoptosis in MPC5 cells. Podocytes were transfected with siRNA against adiponectin or NC siRNA. a, The transfection efficiency was evaluated by qRT-PCR and Western blot. *** p < 0.001 vs. the NC siRNA group. b, CCK-8 assay was used to evaluate cell viability after transfection. ** p < 0.01, *** p < 0.001 vs. controls. c, The percentage of apoptotic cells was determined after the transfection. * p < 0.05, ** p < 0.01,*** p < 0.001 vs. controls. d, The mRNA expressions of nephrin, podocin, desmin, NLRP3, caspase-1, and NF-κB were evaluated by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. controls. e-f, Western Blot analysis of nephrin, podocin, desmin, NLRP3, caspase-1, and NF-κB. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. controls. g, Levels of inflammatory cytokines IL-18 and IL-1β were assessed by ELISA. *** p < 0.001 vs. controls. Data are presented as mean ± SEM from three independent experimentsBack to article page