Fig. 5

The NLRP3 inflammasome inhibitor MCC950 attenuated podocyte injury and intracellular oxidative stress induced by PA treatment or insufficient adiponectin expression. a, CCK-8 assay was performed to evaluate cell viability. * p < 0.05, *** p < 0.001 vs. controls. b, ROS production was assessed using a fluorometry assay. *** p < 0.001 vs. controls. c, The apoptosis was determined by flow cytometry. *** p < 0.001 vs. controls. d, qRT-PCR analysis of nephrin, podocin, desmin, NLRP3, caspase-1, and NF-κB. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. controls. e, Levels of inflammatory cytokines IL-1β and IL-18 were measured by ELISA. *** p < 0.001 vs. controls. f-g, Western blot analysis determined the protein levels of nephrin, podocin, desmin,NLRP3, procaspase-1, cleaved caspase-1, ASC, p65, and phosphorylated p65. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. controls. Data are presented as mean ± SEM from three independent experiments