Fig. 6

Effects of PDTC on NLRP3 inflammasome activation and expression of podocyte makers in PA-stimulated podocytes. Podocytes subjected to adiponectin treatment (0 or 2 µg/mL) were preconditioned with or without 50 µM PDTC for 2 h, followed by exposure to 150 µM PA for 24 h. a, d The levels of NF-κB and p-NF-κB were detected by Western blot. b, e The levels of podocyte makers (nephrin, podocin, and desmin) were determined by Western blot. c, f The levels of NLRP3 inflammasome (NLRP3, cleaved caspase-1, procaspase-1, and ASC) were measured by Western blot. * p < 0.05, *** p < 0.001 vs. the control group. Data are presented as mean ± SEM from three independent experiments