Fig. 1

Clinical findings of this case are shown. A Proteinuria and serum creatinine are shown. B (a) Periodic acid methenamine silver (PAM)-stained kidney sections observe by light microscopy and (b) ultrathin sections observe by electron microscopy reveal that the patient at age 68 has membranous nephropathy stage II. (c and d) Immunohistochemical analysis shows that the specimen of the first renal biopsy is negative for THSD7A. C (a) PAM-stained kidney sections and (b) ultrathin sections reveal that the patient at 72 years has membranous nephropathy stage III. (c and d) Immunohistochemistry shows that the specimen of the second renal biopsy is positive for THSD7A. (scale bars: (a) 50 μm, (b) 5 μm, (c) 50 μm, and (d) 20 μm) Abbreviations: PCI, percutaneous coronary intervention; PSL, prednisolone