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Fig. 3 | BMC Nephrology

Fig. 3

From: Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury

Fig. 3

Characterization of MAbs. (A) The ascites were assessed by SDS-PAGE under non-reducing and reducing, Lane M, protein marker; Lane 1 and 3, purified the ascites of 2F4; Lane 2 and 4, purified the ascites of 1G1.(B) The ascites were assessed by Western blot, Lane 1, Western blot analysis of mAb 2F4; Lane 2, Western blot analysis of mAb 1G1. (C) The Cross-reactivity of 2 hybridoma lines was tested by Indirect ELISA, NGAL-6×His and PCT-6×His were coated onto microtiter plates. The positive control was a NGAL-6×His immune serum; PBS were used as blank control. Red and black dots were meant 1G1 and 2F4 ELISA OD450 nm values, respectively. (D) Relative affinity of MAb 2F4 and 1G1

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BMC Nephrology

ISSN: 1471-2369

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