Renal biomarkers of acute kidney injury in response to increasing intermittent hypoxia episodes in the neonatal rat

Table 1 Quantitative analysis of immunostaining intensities

Variable	ACE (n = 6)	ACE2 (n = 6)	ET-1 (n = 6)	ET-2 (n = 6)	ET_A (n = 6)	ET_B (n = 6)	HIF_1α (n = 12)	TUNEL (n = 12)
*Room Air (RA)*
P14	1161.8± 173.3	1671.0± 566.1	1877.8± 429.7	8254.0± 353.4	2988.5± 396.7	3255.3± 504.2	388.2 ± 60	105.8 ± 15.6
P21	1968.8± 171.8	4523.3± 434.3	1295.8± 35.9	3922.0± 795.1	2652.7± 410.7	3558.5± 310.2	246.7 ± 50	107.7 ± 23.2
*50% O2*
P14	3123.6± 426.0*	1117.0± 32.8	10,158.8± 2408.4**	8041.8± 1998.2	6863.2± 224.5*	951.8± 84.5*	554.0± 136.8	5291.1± 180.6**
P21–7D	2077.2± 189.3	17,920.6± 2485.8**	17,723.5± 3088.7**	2266.8± 496.0	370.8± 38.5**	220.0± 39.5**	5381.0± 1085.6**	3746.2± 490.0**
P21–14D	11,390.7 ± 2356.4*	19,296.2± 2904.2**	12,053.4 ± 1790.1**	8720.2± 798.8**	1649.7± 73.0*	1849.8± 243.9**	4978.7± 1025.4**	3625.0± 723.9**
*Neonatal Intermittent Hypoxia (8 episodes/day)*
P14	3874.0± 816.5**	6731.5± 780.8**	26,629.0± 1495.3**	6473.0± 1333.2	11,053.0± 1523.2**	17,013± 924.4**	4596.2± 959.6**	8191.6± 634.9**
P21–7D	18,342.0 ± 1835.9**	45,587.2± 2696.8**	30,999.3± 4202.0**	40,048.3 ± 5332.7**	4065.0± 246.6**	1453.8± 167.5**	4775.8± 200.4**	2336.4± 605.6**
P21–14D	27,158.0 ± 4041.5**	37,150.7 ± 1561.8**	15,976.4 ± 1611.9**	2207.6± 442.9	2487.2± 111.7	1652.6± 133.2**	11,571.2 ± 3812.0**	3533.0± 152.8**

Data are mean ± SEM (n = number/group; *p < 0.05; **p < 0.01 vs RA). Comparison among the groups for each age category was determined by one-way ANOVA with Dunnett’s multiple comparison post hoc test. Animals were exposed to 14 days of neonatal IH (P14), or placed in room air for recovery for 14 days after 7 days of neonatal IH (P21–7D), or for 7 days after 14 days of neonatal IH (P21–14D)

ISSN: 1471-2369