Fig. 2

Kidney biopsies from proband, 6238, and sibling 6237. A 6238: The first biopsy showed classic segmental sclerosis/hyalinosis lesion on a background of mild mesangial hypercellularity (PAS, 20x). B 6238: Ultrastructural examination showed mild podocyte foot process effacement and normal glomerular basement membranes (GBM)(2500x). C and D 6237: no significant GBM alterations (red arrows) are seen but there are regions of dense extracellular matrix (*), postulated to be mesangial, which appear to enclose cells to the point where only the nucleus is visible (yellow arrows). (E) Comparison of nested averages (mean ± SEM) for each individual: 6237(M): 574.3 nm ± 11.8; 6238(M): 345.7 nm ± 8.1; 6463(F): 375.6 nm ± 8.5; 7825(M): 495.9 nm ± 7.2; all individuals, except 6238(M) & 6463(F), are significantly different from each other (p < 0.0001). Dotted lines are the average GBM thickness for males (red; 373 ± 42 nm) and females (blue; 326 ± 45 nm) according to Steffes et al. Lab Invest. 1983 Jul;49(1):82–6