Table 1 The sequences of the forward and reverse primers and PCR condition
From: The effect of BKV reactivation on cytokines behavior in kidney transplanted patients
Gene name | mRNA ID | Primers | Length (bp) | Annealing temperature (°C) | PCR condition |
|---|---|---|---|---|---|
IRF3 | NM_001197122.2 | Forward: 5′- TTGGGGACTTTTCCCAGCC Reverse: 5′- TCCAGAATGTCTTCCTGGGT | 82 | 58 | 1 cycle: 95 °C /5 min., 40 cycles: 95 °C/30 s 20 s/ annealing 72 °C/30 s Followed by: melt curve |
IRF7 | NM_001572.5 | Forward: 5′- GTGAGGGTGTGTCTTCCCTG Reverse: 5′- TCGTCATAGAGGCTGTTGGC | 73 | ||
GAPDH | NM_001357943.2 | Forward: 5′-GGACTCATGACCACAGTCC Reverse: 5′-CCAGTAGAGGCAGGGATGAT | 119 | ||
IFN-γ | NM_000619.3 | Forward: 5′- CAGCTCTGCATCGTTTTGGG Reverse: 5′- TCCGCTACATCTGAATGACCTG | 110 | ||
TLR3 | NM_003265.3 | Forward: 5′-GGGCAAGAACTCACAGGCCAGG Reverse: 5′-AAGGGCCACCCTTCGGAGCA | 147 | ||
IL-27R | NM_004843.4 | Forward: 5′- CGGAGCTGAAGACCATACCC Reverse: 5′- CGCCCGACAAATCCTCTTCT | 114 | 59 | |
TNFARB | NM_001066.3 | Forward: 5′- CACATGCCGGCTCAGAGAAT Reverse: 5′- AGCTGGGTGTATGTGCTGTC | 144 | ||
TLR4 | NM_003266.4 | Forward: 5′- TCAAGCCAGGATGAGGACTGGGT Reverse: 5′- CAGCAATGGCCACACCGGGA | 118 | ||
IL-27 | NM_145659.3 | Forward: 5′- GTGAACCTGTACCTCCTGCC Reverse: 5′- CGTGGTGGAGATGAAGCAGA | 111 | 60 | |
TNFARA | NM_001065.4 | Forward: 5′- GAGAGGCCATAGCTGTCTGG Reverse: 5′-CTCTCACACTCCCTGCAGTC | 124 | ||
TNF-α | NM_001065.4 | Forward: 5′-CTTCTGCCTGCTGCACTTTG Reverse: 5′- CTACAGGCTTGTCACTCGGG | 128 | 61 |