Fig. 3From: Thrombotic microangiopathy with transiently positive direct Coombs test in an adult with poststreptococcal acute glomerulonephritis: a case reportEvaluation of glomerular damage by histological staining of fresh frozen tissue sections showing the positive glomerular staining of nephritis-associated plasmin receptor (NAPlr) and plasmin activity in simillar fashion, supporting the diagnosis of PSAGN. a NAPlr was positive by IF staining, mainly in the inner side of glomerular tufts (fluorescein isothiocyanate [FITC], green). b C3 staining on the same section was distributed mainly along the capillary walls (Alexa Fluor 594 [AF594], red). c A merged image demonstrating the different localizations of NAPlr and C3. d In situ zymography for plasmin activity in an adjacent section to (a) showed positive staining in the glomerulus with a similar distribution to NAPlr staining. Scale bars = 20.0 μm in (a)-(d)Back to article page