Fig. 5

Multiple staining for CD3, CD68, T-antigen, and neutrophils on a fresh frozen biopsy section showing the relationship between T-antigen exposed site and the inflammatory cells infiltration. a Indirect IF staining for CD3 (Alexa Fluor 594: red) and (b) CD68 (Alexa Fluor 350: blue), (c) direct staining with FITC-conjugated peanut lectin (T-antigen: green), and (e) chloroesterase staining (light microscopy, blue) were performed on a section of fresh frozen renal tissue from the patient. A merged image of the staining results (d) revealed the massive accumulation of CD3-positive T-cells and CD68-positive macrophages around the peanut lectin-positive tubulointerstitial lesions, although infiltration of chloroesterase-positive neutrophils was rare (e). Scale bars = 20.0 μm in (a)-(e)