Fig. 1

Antibody reactivities in pre-second transplant serum against DP single antigen panel beads. The reactions against the E residue at amino acid position 56 (56E eplet) in DPB1 and against the Q residue at amino acid position 50 (50Q eplet) in DPA1 are shown at the bottom and highlighted in the dashed boxes. DPB1*03:01 in the first donor and DPB1*02:01 in the second donor share the 56E eplet and are underlined. The mean fluorescence intensity cutoff value for a positive result was set at 1000. Beads carrying the patient’s DPA1 or DPB1 alleles are labeled as self, while beads carrying the donors’ DPA1 or DPB1 alleles are indicated as imm (abbreviation for immunizer). Because DPA1*02:01 and DPA1*02:02 have identical allogeneic eplet profiles, DPA1*02:01 is used as a substitute for the patient’s allele. The positive reactions against DR11 alleles are due to cross-reactions with E residue at amino acid position 58 in DR11