Fig. 2

Mibefradil inhibits phosphorylation of ERK1/2 in the presence of 5%, FBS, PDGF or TGFβ1. A representative Western blot showing phosphorylated ERK1/2 levels in MES13 cells with and without mibefradil treatment in 1% FBS (a) for 4 h, in all media conditions (with 5% FBS, PDGF or TGFβ1). The full-length images of Western blot are presented in Supplementary figure S5. (b) shows the mean densitometry quantification of pERK1/2 levels (normalised to total ERK1/2) in three independent experiments with MES13 cells treated with mibefradil for 4 h and controls in a range of media conditions (5% FBS, PDGF or TGFβ1). The data are represented as mean ± SD, n = 3, ****p < 0.0001, from a One-Way ANOVA with Bonferroni post hoc correction