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Fig. 2 | BMC Nephrology

Fig. 2

From: Cyclosporine therapy could be considered for membranoproliferative glomerulonephritis with immunoglobulin A deposits: a case report

Fig. 2

Immunofluorescent studies of the first and second renal biopsies. Fluorescence microscope microscopy Nikon ECLIPSE Ts2R and NIS Elements (Nikon Corporation, Japan) is used to capture the images. The images were obtained with eyepiece at 10X magnification and objective at 20X. The brightness and contrast were adjusted by using Phtoshop (adobe, Japan). No other downstream processing or averaging were applied to the images to enhance the resolution. On the first biopsy (a-d), positive deposits of IgA (a) and C3 (d) were dominantly detected in the mesangial regions, and were also slightly detected along the capillary walls. In addition, IgA deposits were stronger than those of C3. IgA2 (c) staining was stronger than IgA1 (b) staining. On the second biopsy (eh), positive deposits of IgA (e) were detected mainly in the mesangial regions and also slightly along the capillary walls. C3 (h) deposits were not detected. IgA1 (f) deposits were not found, and IgA2 (g) deposits were found in the mesangial region. (Magnification: × 200, a-h)

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