Table 1 PCR Primers of the respiratory tract virus
Virus | Primer sequence(5'-3') | Product(bp) | |
|---|---|---|---|
Adenovirus | ADV-1 | 5'-CAGGACGCCTCGGAGTACCTGAG-3' | 103 |
ADV-2 | 5'-GGAGCCACGGTGGCGCC-3' | ||
Rhinovirus | RHV-1 | 5'-CGGACACCCAAAGTAG-3' | 380 |
RHV-2 | 5'-GCACTTCTGTTTCCCC-3' | ||
Parainfluenza Virus | PFV1-F | 5'-GTTGTCAATGTCTTAATTCGTATCAATAATT-3' | 108 |
PFV1-R | 5'-GTAGCCTACCTTCGGCAC-3' | ||
PFV2-F | 5'-GCATTTCCAATCTTCAGGACTATGA-3' | 89 | |
PFV2-R | 5'-ACCTCCTGGTATAGCAGTGACTGAAC-3' | ||
PFV3-F | 5'-AGTCATGTTCTCTAGCACTCCTAAATACA-3' | 135 | |
PFV3-R | 5'-ATTGAGCCATCATAATTGACAATATCAA-3' | ||
Influenza Virus | INFA-F | 5'-GGACTGCAGCGTAGACGCTT-3' | 188 |
INFA-R | 5'-CATCCTGTTGTATATGAGGCCCAT-3' | ||
INFB-F | 5'-AAGTACGGTGGATTAAATAAAAGCAA-3' | 169 | |
INFB-R | 5'-CCAGCAATAGCTCCGAAGAAA-3' | ||
Coronavirus | CV229E-F | 5'-TGGCCCCATTAAAAATGTGT-3' | 575 |
CV229E-R | 5'-CCTGAACACCTGAAGCAAT-3' | ||
CVOc43-F | 5'-GGCTTATGTGGCCCCTTACT-3' | 334 | |
CVOc43-R | 5'-GGCAAATCTGCCCAAGAATA-3' | ||
CVNL63-F | 5'-CTGTTACTTTGGCTTTAAAGAACTTAGG-3' | 314 | |
CVNL63-R | 5'-CTCACTATCAAAGAATAACGCAGCCTG-3' | ||
CVHKU1-F | 5'-ACCAATCTGAGCGAAATTACCAAAC-3' | 443 | |
CVHKU1-R | 5'-CGGAAACCTAGTAGGGATAGCTT-3' | ||
Respiratory Syncytial Virus | RSVAB-F | 5'-GGAAACATACGTGAACAAGCTTCA-3' | 80 |
RSVA-R | 5'-CATCGTCTTTTTCTAAGACATTGTATTGA-3' | ||
RSVB-R | 5'-TCATCATCTTTTCTAGAACATTGTACTGA-3' | 81 | |
GAPDH | GAPDH-F | 5'-CGCTCTCTGCTCCTCCTGT-3' | 81 |
GAPDH-R | 5'-CCATGGTGTCTGAGCGATGT-3' |