Precise diagnosis and typing of early-stage renal immunoglobulin-derived amyloidosis by label-free quantification of parallel reaction monitoring-based targeted proteomics

Table 2 Verification of PRM the sensitivity and specificity, area under the curve, and the combined effect to assess the diagnostic abilities of peptides and peptide combinations derived from amyloidogenic proteins to type renal amyloidosis

Protein Name	Peptide Sequence	Charge	Precursor Ions m/z	Retention Time Window [min]	Peptide AUC	Protein AUC
Apolipoprotein E	AATVGSLAGQPLQER	+ +	749.404629	10–17	0.8653	0.9733
Apolipoprotein E	SELEEQLTPVAEETR	+ +	865.925788	13.2–20.3	0.8584
Apolipoprotein E	VEQAVETEPEPELR	+ +	813.404491	8.9–15.9	0.828
Serum amyloid P-component	AYSLFSYNTQGR	+ +	703.83859	14.2–21.2	0.8109	0.9815
Serum amyloid P-component	IVLGQEQDSYGGK	+ +	697.351531	7.2–14.2	0.8182
Serum amyloid P-component	VGEYSLYIGR	+ +	578.803488	13.3–20.3	0.8174
Apolipoprotein A-IV	LAPLAEDVR	+ +	492.279649	8.2–15.2	0.79	0.9339
Apolipoprotein A-IV	LEPYADQLR	+ +	552.787838	8.5–15.5	0.7681
Apolipoprotein A-IV	SLAPYAQDTQEK	+ +	675.830431	5.1–12.1	0.815
Immunoglobulin lambda light chain	AAPSVTLFPPSSEELQANK	+ +	993.512562	17.3–24.3	0.8524	0.9227
Immunoglobulin lambda light chain	ADGSPVNTGVETTTPSK	+ +	830.904856	14–18	0.6502
Immunoglobulin lambda light chain	SGTSASLAISGLR	+ +	610.335684	8–12	0.7214
Immunoglobulin kappa light chain	TVAAPSVFIFPPSDEQLK	+ +	973.517116	22–29	0.8926	0.8926
Immunoglobulin gamma-1 heavy chain	GPSVFPLAPSSK	+ +	593.826963	13–20	0.8546	0.8621
Immunoglobulin gamma-1 heavy chain	TTPPVLDSDGSFFLYSK	+ +	937.464549	21.9–28.9	0.7445

ISSN: 1471-2369