Table 3 the most stable 4 internal standard proteins and corresponding peptides validated by LMD/PRM-MS based targeted proteomics
Protein Name | Peptide Sequence | Charge | Precursor Ions m/z | Retention Time Window [min] | CV% |
|---|---|---|---|---|---|
Histone H4 | DNIQGITKPAIR |  +  +  | 663.380425 | 6.9–13.9 | 20.19 |
Histone H4 | ISGLIYEETR |  +  +  | 590.814053 | 10.7–17.7 | 27.98 |
Prelamin-A/C | NSNLVGAAHEELQQSR |  +  +  | 876.934813 | 7.7–14.7 | 33.08 |
Vimentin | TYSLGSALRPSTSR |  +  +  | 748.396804 | 8.1–15.1 | 29.23 |
Vinculin | AVAGNISDPGLQK |  +  +  | 635.343509 | 6.3–13.3 | 37.32 |
Vinculin | SLGEISALTSK |  +  +  | 553.308603 | 11.8–18.8 | 32.95 |