Mixed infection of three nontuberculous mycobacteria species identified by metagenomic next-generation sequencing in a patient with peritoneal dialysis-associated peritonitis: a rare case report and literature review

Table 1 Results of three primer pairs used to specifically amplify target fragments

Primer	Sequence(5′ → 3′)	Target fragment (bp)	Species
MS-2F1	GGTTCCCGAACTGAACTGCC	322	Mycobacterium smegmatis
MS-2R1	CACCACGGTCAAGACGATGAA
MS-2F2	ATGCGGAAGACGACTGGG	308
MS-2R2	TGGGTGCTTACTGACTTGAATG
MS-F1	TACAGGCCTTTCGAGGATCC	148
MS-R1	GGTACTCCGAACGATGATCCT
MG-2F1	GTGCCATGTCCTACCTGAAGAG	229	Mycobacterium goodi
MG-2R1	TGGTCGGGCTTGCTCCTA
MG-2F2	GGAACGACGCAACGGTAGA	840
MG-2R2	CACTGATGGACGCCTTGAGTAA
MG-F1	AGTTCATCATGGTCGATCGC	150
MG-R1	CATGACCAAGCCGTTCCAC
MA-2F1	GCCGACGAGACCAACATCC	1061	Mycobacteroides abscessus
MA-2R1	CAGTGCTCACCCTTGCCAGTA
MA-2F2	CGAATCCACCACGATGACG	320
MA-2R2	GTCGAGGACGATCTTGAGCC
MA-F1	GTTGCGATCTAGGTGTGCTG	133
MA-R1	TCAAAGTACCGCTCAATCGC

ISSN: 1471-2369