Fig. 3

Immunostaining for nephrin(green) and claudin-1(red), in normal control and extra-capillary cell proliferation in this case. a Nephrin was expressed in podocytes and claudin-1 was expressed in PECs in normal control (0 h biopsy of transplant graft). b Nephrin was down-regulated in the capillary loop. c Claudin-1 was strongly positive in extra-capillary cell proliferations. d Merged (b) and (c). Nephrin and claudin-1 was co-stained in the area surrounded by the lines