Fig. 1
From: KLHL3-dependent WNK4 degradation affected by potassium through the neddylation and autophagy pathway
The neddylation and autophagy in the KLHL3-dependent WNK4 degradation. a Immunoblotting was performed with antibodies against WNK4, pWNK4, KLHL3-myc, NEDD8 ,and cullin3. Compared with control groups, the expression of WNK and pWNK4 increases significantly in the experimental groups. Densitometry analysis of WNK4, pWNK4, KLHL3, Nedd8 and Cullin3 . **P < 0.01, *P < 0.05 n= 6 b HEK293T cells were treated with an autophagy inhibitor (3MA) and MLN4924 separately and in combination for 24 h. The combined effect of 3MA and MLN4924 for WNK is stronger than the single effect. Densitometry analysis of WNK4, pWNK4, KLHL3, NEDD8 ,and LC3B.****P <0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 n= 4 c Another autophagy inhibitor, Bafilomycin A1, is similar to 3MA