Fig. 2
From: KLHL3-dependent WNK4 degradation affected by potassium through the neddylation and autophagy pathway
Potassium regulates WNK4 via autophagy and apoptosis. HEK293 cells co-expressed with KLHL3 and WNK4 kinase were treated by different potassium ion concentrations shown in a and b. It shows that the abundance of WNK4，pWNK4，NEDD8-Cullin3,capase3 ,and LC3B II increases in LK groups more than in HK groups. The expression of WNK4 and pWNK4 is higher with two drugs (MLN4924 and Bafilomycin A1) than MLN4924 only. Densitometry analysis of WNK4, pWNK4, KLHL3, NEDD8, CUL3, P62, KEAP1 and LC3B. ****P <0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 n= 4