Fig. 2
PF attenuated intracellular ROS generation in H/R-induced HK-2 cells. HK-2 cells were pre-treated with 50, 100, and 200µM PF for 4 h, incubated with DMEM/F12 (serum-free culture medium) containing 400µM of COCL2 for 24 h to mimic a hypoxia environment, and then subjected to reoxygenation treatment for 4 h. The intracellular ROS levels were directly observed under a fluorescence microscope after DCFH-DA loading. (A) Intracellular ROS were detected by fluorescence microscopy. (B) The results are presented as the mean fluorescence intensity as analyzed by Image J software. Data are expressed as the mean ± SD (n = 3). ****p < 0.0001, in comparison with the NC group. #p < 0.05, ####p < 0.0001, in comparison with the H/R group. NC, normal control, H/R, hypoxia/reoxygenation, PF, paeoniflorin, SD, standard deviation