Fig. 3

PF decreased apoptosis and the expression of HIF-1α in H/R-induced HK-2 cells. HK-2 cells were pre-processed by 200µM of PF for 4 h and then subjected to H/R treatment as mentioned earlier. The grouping of blots cropped from different parts of the same gel. (A) The protein expression of HIF-1α was detected by Western blotting. (B) The protein expression of Bax and Bcl-2 were measured by Western blotting. (C–E) The changes in the protein expression of HIF-1α, Bax, and Bcl-2 were standardized to those of β-actin. The values are expressed as the mean ± SD (n = 3). **p < 0.01, in comparison with the NC group. ##p < 0.01, in comparison with the H/R group. NC, normal control, H/R, hypoxia/reoxygenation, PF, paeoniflorin, SD, standard deviation