Fig. 5
The role of PF in the regulation of the Keap1/Nrf2/HO-1 pathway. HK-2 cells were pre-processed by 200µM of PF for 4 h and then subjected to H/R treatment as mentioned earlier. The grouping of blots cropped from different parts of the same gel. (A) The expression of Keap1, HO-1, and Nrf2 were measured by Western blotting. (B–D) The changes in the protein expression of Keap1, HO-1, and Nrf2 were standardized to those of β-actin. The data are expressed as the mean ± SD (n = 5). **p < 0.01, ****p < 0.0001, in comparison with the NC group. ##p < 0.01, in comparison with the H/R group. HO-1, heme oxygenase 1, Nrf2, nuclear factor erythroid 2-related factor 2, Keap1, Kelch-like ECH-associated protein 1, NC, normal control, H/R, hypoxia/reoxygenation, PF, paeoniflorin, SD, standard deviation